Purification of recombinant proteins tagged with histidine can be charged with other transition metals IDA, provided charged with Ni 2+ and uncharged Pressure-stable polymer based on UNOsphere beads Purification of recombinant histidine-tagged proteins can be charged with other transition metals High capacity, pressure-stable polymer based on UNOsphere™ beads Ready to Use Affinity Media Selection Guide Ready-to-Use Affinity Media Theoretically, any protein can be purified using the tagging method however, many factors must be considered to design a process to purify tagged recombinant proteins.īio-Rad offers ready to use affinity media and customizable or activated media. Two of the most commonly used protein tags are the polyhistidine tag, which binds to certain metal-containing complexes such as those in Profinity™ IMAC resins, and the glutathione s-transferase (GST) sequence, which binds to glutathione, found in Bio-Scale™ Mini Profinity™ GST media. A number of different tags are available.